Plasmid

Part:BBa_K4895202:Design

Designed by: Patrick Jiang, Rori Hoover   Group: iGEM23_ASU   (2023-10-12)


mScarlet Optogentically controlled plasmid backbone (N-terminally His tagged)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1103
    Illegal NgoMIV site found at 1235
    Illegal NgoMIV site found at 1329
    Illegal NgoMIV site found at 1622
    Illegal NgoMIV site found at 2116
    Illegal NgoMIV site found at 2134
    Illegal NgoMIV site found at 2224
    Illegal AgeI site found at 1454
    Illegal AgeI site found at 2582
    Illegal AgeI site found at 4451
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Below is a review of the major design considerations

  1. Lac operon promoter and Lac operon terminator to ensure expression of mScarlet placed inside the Bbs1 cut sites
  2. T7 promoter, T7 terminator and T7 tag placed outside of the Bbs1 cut sites
  3. Kanamycin resistance
  4. FixLJ/K2 - non-coding regions that enforce optogenetic control of the plasmid


Source

This part contains portions of genomic sequences. The insert mScarlet, as well as Kanamycin resistance is not native to e.coli. Furthermore, the optogenetic control of the part is not native to e.coli either.

References