Plasmid
Part:BBa_K4895202:Design
Designed by: Patrick Jiang, Rori Hoover Group: iGEM23_ASU (2023-10-12)
mScarlet Optogentically controlled plasmid backbone (N-terminally His tagged)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1103
Illegal NgoMIV site found at 1235
Illegal NgoMIV site found at 1329
Illegal NgoMIV site found at 1622
Illegal NgoMIV site found at 2116
Illegal NgoMIV site found at 2134
Illegal NgoMIV site found at 2224
Illegal AgeI site found at 1454
Illegal AgeI site found at 2582
Illegal AgeI site found at 4451 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Below is a review of the major design considerations
- Lac operon promoter and Lac operon terminator to ensure expression of mScarlet placed inside the Bbs1 cut sites
- T7 promoter, T7 terminator and T7 tag placed outside of the Bbs1 cut sites
- Kanamycin resistance
- FixLJ/K2 - non-coding regions that enforce optogenetic control of the plasmid
Source
This part contains portions of genomic sequences. The insert mScarlet, as well as Kanamycin resistance is not native to e.coli. Furthermore, the optogenetic control of the part is not native to e.coli either.